Journal: Biotechnology and bioengineering

Article Title: Mesenchymal stem cells and local tacrolimus delivery synergistically enhance neurite extension

doi: 10.1002/bit.27916

Figure Lengend Snippet: Presence and verification of stem cells is visualized after seeding with mesenchymal stem cells (MSC; 2-IB, 2-IIB), treatment with 100 ng/mL tacrolimus (FK506; 2-IC, 2-IIC), or MSCs and 100 ng/mL FK506 combined (2-ID, 2-IID), and compared to control (2-IA, 2-IIA; no treatment). Neurites grew from the DRG into the nerve graft: from the circular top to bottom, respectively. Presence of the CD90 marker (green) in both autograft groups and allograft groups was evident on the nerve grafts seeded with MSCs and nerve grafts with combined treatment (respectively, groups B and D).

Article Snippet: After CD90 staining (monoclonal mouse anti rat 1:100, Bio-Rad, Hercules, CA, USA), sections are incubated at RT for 2 hours with DAPI (4’,6- Diamidino-2-Phenylindole, Dihydrochloride) (1:1000, Thermo Fisher Scientific).

Techniques: Control, Marker

Journal: BMC Nephrology

Article Title: Epoetin beta pegol for treatment of anemia ameliorates deterioration of erythrocyte quality associated with chronic kidney disease

doi: 10.1186/s12882-018-0818-4

Figure Lengend Snippet: Time course of Hb and RBC count. Hb levels ( a ) and RBC numbers ( b ) progressively and significantly decreased in the UNX-Thy1 + Vehicle group compared with levels in the Sham group. a C.E.R.A. treatment in the UNX-Thy1 group significantly increased Hb to levels nearly the same as those in the Sham group. b RBC number was also significantly increased by C.E.R.A. treatment in the UNX-Thy1 group. Values are shown as mean ± SEM. *, P < 0.05 ( a , b , two-way ANOVA followed by Tukey’s test). Sham, n = 5; UNX-Thy1 + Vehicle, n = 6; UNX-Thy1 + C.E.R.A., n = 6

Article Snippet: Briefly, after left kidney nephrectomy, an anti-rat CD90 (Thy1.1) monoclonal antibody (Cedarlane Labs, Burlington, Canada) at a single dose of 0.6 mg/kg body weight was intravenously injected into rats (UNX-Thy1 group, n = 12).

Techniques:

Journal: BMC Nephrology

Article Title: Epoetin beta pegol for treatment of anemia ameliorates deterioration of erythrocyte quality associated with chronic kidney disease

doi: 10.1186/s12882-018-0818-4

Figure Lengend Snippet: Plasma oxidative stress. The level of d-ROMs was significantly increased in the plasma of the UNX-Thy1 + Vehicle group compared with the Sham group. C.E.R.A. treatment in the UNX-Thy1 group tended to decrease the levels of plasma d-ROMs. Values are shown as mean ± SEM. *, P < 0.05 (Tukey’s test). Sham, n = 5; UNX-Thy1 + Vehicle, n = 6; UNX-Thy1 + C.E.R.A., n = 6

Article Snippet: Briefly, after left kidney nephrectomy, an anti-rat CD90 (Thy1.1) monoclonal antibody (Cedarlane Labs, Burlington, Canada) at a single dose of 0.6 mg/kg body weight was intravenously injected into rats (UNX-Thy1 group, n = 12).

Techniques: Clinical Proteomics

Journal: BMC Nephrology

Article Title: Epoetin beta pegol for treatment of anemia ameliorates deterioration of erythrocyte quality associated with chronic kidney disease

doi: 10.1186/s12882-018-0818-4

Figure Lengend Snippet: Erythrocyte deformability. Erythrocyte deformability is shown as the value of the elongation index. a Compared with the stable erythrocyte deformability status in the Sham group, erythrocytes in the UNX-Thy1 + Vehicle group showed a significant deterioration of deformability. C.E.R.A. treatment in UNX-Thy1 group significantly improved erythrocyte deformability. b A significant negative correlation was observed between MCV and erythrocyte deformability. Values are shown as mean ± SEM. *, P < 0.05 ( a , two-way ANOVA followed by Tukey’s test; b , Pearson’s correlation test). Sham, n = 5; UNX-Thy1 + Vehicle, n = 6; UNX-Thy1 + C.E.R.A., n = 6

Article Snippet: Briefly, after left kidney nephrectomy, an anti-rat CD90 (Thy1.1) monoclonal antibody (Cedarlane Labs, Burlington, Canada) at a single dose of 0.6 mg/kg body weight was intravenously injected into rats (UNX-Thy1 group, n = 12).

Techniques:

Journal: BMC Nephrology

Article Title: Epoetin beta pegol for treatment of anemia ameliorates deterioration of erythrocyte quality associated with chronic kidney disease

doi: 10.1186/s12882-018-0818-4

Figure Lengend Snippet: Erythrocyte turnover. a Biotinylated erythrocytes significantly decreased in the UNX-Thy1 + Vehicle group compared with in the Sham group. C.E.R.A. treatment in the UNX-Thy1 group significantly suppressed the decrease in biotinylated erythrocytes in circulation. b Although there was no statistical significance, total bilirubin in the plasma of the UNX-Thy1 + Vehicle group tended to increase compared with that in the Sham group. C.E.R.A. treatment in the UNX-Thy1 group tended to decrease plasma total bilirubin. Values are shown as mean ± SEM. *, P < 0.05 ( a , two-way ANOVA followed by Tukey’s test; b , Tukey’s test). Sham, n = 5; UNX-Thy1 + Vehicle, n = 6; UNX-Thy1 + C.E.R.A., n = 6

Article Snippet: Briefly, after left kidney nephrectomy, an anti-rat CD90 (Thy1.1) monoclonal antibody (Cedarlane Labs, Burlington, Canada) at a single dose of 0.6 mg/kg body weight was intravenously injected into rats (UNX-Thy1 group, n = 12).

Techniques: Clinical Proteomics

Journal: BMC Nephrology

Article Title: Epoetin beta pegol for treatment of anemia ameliorates deterioration of erythrocyte quality associated with chronic kidney disease

doi: 10.1186/s12882-018-0818-4

Figure Lengend Snippet: Intracellular calcium level in erythrocytes. a Intracellular calcium level as assessed by Fluo-3/AM intensity was significantly increased in erythrocytes in the UNX-Thy1 + Vehicle group compared with those in the Sham group. C.E.R.A. treatment in the UNX-Thy1 group significantly decreased intracellular calcium level to nearly the same level as in the Sham group. b A significant negative correlation was observed between intracellular calcium level in erythrocytes and erythrocyte deformability. Values are shown as mean ± SEM. *, P < 0.05 ( a , Tukey’s test; b , Pearson’s correlation test). Sham, n = 5; UNX-Thy1 + Vehicle, n = 6; UNX-Thy1 + C.E.R.A., n = 6

Article Snippet: Briefly, after left kidney nephrectomy, an anti-rat CD90 (Thy1.1) monoclonal antibody (Cedarlane Labs, Burlington, Canada) at a single dose of 0.6 mg/kg body weight was intravenously injected into rats (UNX-Thy1 group, n = 12).

Techniques:

Journal: Laboratory investigation; a journal of technical methods and pathology

Article Title: The oncofetal protein glypican-3 is a novel marker of hepatic progenitor/oval cells.

doi: 10.1038/labinvest.3700479

Figure Lengend Snippet: Figure 5 mRNA expression analysis of isolated Thy1 positive progenitor and PDGFR-b positive stellate cells. Thy1 þ cells were isolated from 2-AAF/PH liver at day 10 after surgery and PDGFR- b þ cells were isolated from D-gal-treated liver. (a) RT-PCR analysis of AFP, CD45, CK-19, GAPDH, Gpc3 and Thy1 mRNA expression in FACS sorted Thy1 positive/CD45 negative progenitor cells from 2-AAF/PH liver. (b) RT-PCR analysis of AFP, CD45, CK-19, desmin, GAPDH, and Gpc3 mRNA expression in PDGFR-b positive stellate cells isolated with microbeads from D-gal-treated liver.

Article Snippet: For cell sorting monoclonal anti-rat CD45 PE (cat num CL009PE) and anti-rat Thy1 (CD90) FITC (cat num CL005F) were purchased from Cedarlane (Burlington, NC, USA) and used at dilution 1:50.

Techniques: Expressing, Isolation, Reverse Transcription Polymerase Chain Reaction